dlbcas12a sequence (Addgene inc)
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dlbcas12a sequence
Dlbcas12a Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dlbcas12a/pPN10-gCTG+(Plasmid+%2384774)/bio_rxiv__2024__07__08__602263-266-9-16
Average 92 stars, based on 1 article reviews
Dlbcas12a Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dlbcas12a/pPN10-gCTG+(Plasmid+%2384774)/bio_rxiv__2024__07__08__602263-266-9-16
Average 92 stars, based on 1 article reviews
dlbcas12a sequence - by Bioz Stars,
2026-09
92/100 stars
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Related Articles
Sequencing:Article Title: CRISPR-mediated activation of biosynthetic gene clusters for bioactive molecule discovery in filamentous fungi Article Snippet: To clone dSpCas9-VPR it was amplified from pAG414GPD-dCas9-VPR , which was a gift from George Church (Addgene plasmid #63801). .. The coding sequence of Mutagenesis:Article Title: CRISPR-mediated activation of biosynthetic gene clusters for bioactive molecule discovery in filamentous fungi Article Snippet: To clone dSpCas9-VPR it was amplified from pAG414GPD-dCas9-VPR , which was a gift from George Church (Addgene plasmid #63801). .. The coding sequence of Amplification:Article Title: CRISPR-mediated activation of biosynthetic gene clusters for bioactive molecule discovery in filamentous fungi Article Snippet: To clone dSpCas9-VPR it was amplified from pAG414GPD-dCas9-VPR , which was a gift from George Church (Addgene plasmid #63801). .. The coding sequence of Plasmid Preparation:Article Title: CRISPR-mediated activation of biosynthetic gene clusters for bioactive molecule discovery in filamentous fungi Article Snippet: To clone dSpCas9-VPR it was amplified from pAG414GPD-dCas9-VPR , which was a gift from George Church (Addgene plasmid #63801). .. The coding sequence of Article Title: Mitochondrial import, health and mtDNA copy number variability seen when using type II and type V CRISPR effectors. Article Snippet: .. Plasmid sequences are available on request: pD1301-AD (DNA2.0), for expression of NLSSpyCas9-GFP or COX8ASpyCas9-GFP and unmodified Cas9 gRNA template generation; pEX-A2 (Eurofins), for 5′RP/5′FD gRNA template generation; pSP1(Siksnys lab (Szczelkun et al., 2014)), used as a plasmid cleavage assays substrate plasmid; pEGFP (Clontech), for Jo ur na l o f C el l S ci en ce • A cc ep te d m an us cr ip t expression of COX8A/ATG4D-(SaCas9/LbCas12a/AsCas12a)-GFP or LbCas12a-GFP; Su9GFP (Addgene, #23214 (Chen et al., 2003)), for expression of Su9(SaCas9/LbCas12a/AsCas12a/PI)-GFP and Su9-(mutant/dLbCas12a)-GFP; pLVX-puro (Clontech), used for stable expression of Su9-(LbCas12a)-GFP; PLKO.1 (Addgene, #10878 (Moffat et al., 2006)), for expression of Cas12a crRNAs; SaCas9, LbCas12a, AsCas12a, Article Title: Variability in mitochondrial import, mitochondrial health and mtDNA copy number using Type II and Type V CRISPR effectors Article Snippet: .. Plasmid sequences are available on request: pD1301-AD (DNA2.0), for expression of NLS-SpyCas9-GFP or COX8a-SpyCas9-GFP and unmodified Cas9 gRNA template generation; pEX-A2 (Eurofins), for 5′RP/5′FD gRNA template generation; pSP1(Siksnys lab [ ]), used as a plasmid cleavage assays substrate plasmid; pEGFP (Clontech), for expression of COX8a/ATG4D-(SaCas9/LbCas12a/AsCas12a)-GFP or LbCas12a-GFP; Su9-GFP (Addgene, #23214 [ ]), for expression of Su9-(SaCas9/LbCas12a/AsCas12a/PI)-GFP and Su9-(mutant/dLbCas12a)-GFP; pLVX-puro (Clontech), used for stable expression of Su9-(LbCas12a)-GFP; PLKO.1 (Addgene, #10878 [ ]), for expression of Cas12a gRNAs; SaCas9, LbCas12a, AsCas12a, Construct:Article Title: CRISPR-mediated activation of biosynthetic gene clusters for bioactive molecule discovery in filamentous fungi Article Snippet: To clone dSpCas9-VPR it was amplified from pAG414GPD-dCas9-VPR , which was a gift from George Church (Addgene plasmid #63801). .. The coding sequence of Expressing:Article Title: CRISPR–Cas12a base editors confer efficient multiplexed genome editing in rice Article Snippet: All target sites (Supplemental Table 3) were synthesized as single-strand oligonucleotides by GeneWiz. .. The forward and reverse primers were phosphorylated, annealed, and ligated at the BsmBI site with Instant Sticky-end Ligase Master Mix (New England Biolabs®) into pYPQ141-ZmUBI-RZ-Lb (Addgene #86197) for crRNA expression to be coupled with Article Title: CRISPR–Cas12a base editors confer efficient multiplexed genome editing in rice Article Snippet: All target sites (Supplemental Table 3) were synthesized as single-strand oligonucleotides by GeneWiz. .. The forward and reverse primers were phosphorylated, annealed, and ligated at the BsmBI site with Instant Sticky-end Ligase Master Mix (New England Biolabs®) into pYPQ141-ZmUBI-RZ-Lb (Addgene #86197) for crRNA expression to be coupled with Article Title: Mitochondrial import, health and mtDNA copy number variability seen when using type II and type V CRISPR effectors. Article Snippet: .. Plasmid sequences are available on request: pD1301-AD (DNA2.0), for expression of NLSSpyCas9-GFP or COX8ASpyCas9-GFP and unmodified Cas9 gRNA template generation; pEX-A2 (Eurofins), for 5′RP/5′FD gRNA template generation; pSP1(Siksnys lab (Szczelkun et al., 2014)), used as a plasmid cleavage assays substrate plasmid; pEGFP (Clontech), for Jo ur na l o f C el l S ci en ce • A cc ep te d m an us cr ip t expression of COX8A/ATG4D-(SaCas9/LbCas12a/AsCas12a)-GFP or LbCas12a-GFP; Su9GFP (Addgene, #23214 (Chen et al., 2003)), for expression of Su9(SaCas9/LbCas12a/AsCas12a/PI)-GFP and Su9-(mutant/dLbCas12a)-GFP; pLVX-puro (Clontech), used for stable expression of Su9-(LbCas12a)-GFP; PLKO.1 (Addgene, #10878 (Moffat et al., 2006)), for expression of Cas12a crRNAs; SaCas9, LbCas12a, AsCas12a, Article Title: Variability in mitochondrial import, mitochondrial health and mtDNA copy number using Type II and Type V CRISPR effectors Article Snippet: .. Plasmid sequences are available on request: pD1301-AD (DNA2.0), for expression of NLS-SpyCas9-GFP or COX8a-SpyCas9-GFP and unmodified Cas9 gRNA template generation; pEX-A2 (Eurofins), for 5′RP/5′FD gRNA template generation; pSP1(Siksnys lab [ ]), used as a plasmid cleavage assays substrate plasmid; pEGFP (Clontech), for expression of COX8a/ATG4D-(SaCas9/LbCas12a/AsCas12a)-GFP or LbCas12a-GFP; Su9-GFP (Addgene, #23214 [ ]), for expression of Su9-(SaCas9/LbCas12a/AsCas12a/PI)-GFP and Su9-(mutant/dLbCas12a)-GFP; pLVX-puro (Clontech), used for stable expression of Su9-(LbCas12a)-GFP; PLKO.1 (Addgene, #10878 [ ]), for expression of Cas12a gRNAs; SaCas9, LbCas12a, AsCas12a, |